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computer-assisted measurement through digimizer image analysis software version 4.2.2  (MedCalc Software Ltd)

 
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    Structured Review

    MedCalc Software Ltd computer-assisted measurement through digimizer image analysis software version 4.2.2
    Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed <t>(Digimizer</t> 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).
    Computer Assisted Measurement Through Digimizer Image Analysis Software Version 4.2.2, supplied by MedCalc Software Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer-assisted+measurement+through+digimizer+image+analysis+software+version+4%2E2%2E2/computer+assisted+measurement+through+digimizer+image+analysis+software+version+4+2+2/pmc06372585-117-5-14
    Average 90 stars, based on 1 article reviews
    computer-assisted measurement through digimizer image analysis software version 4.2.2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Three Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease"

    Article Title: Three Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-38646-z

    Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed (Digimizer 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).
    Figure Legend Snippet: Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed (Digimizer 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).

    Techniques Used: Transmission Assay, Electron Microscopy

    Related Articles

    Software:

    Article Title: Three Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease
    Article Snippet: .. EM assessment was performed using computer-assisted measurement through Digimizer (version 4.2.2) image analysis software (MedCalc Software, Ostend, Belgium). ..



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    MedCalc Software Ltd computer-assisted measurement through digimizer image analysis software version 4.2.2
    Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed <t>(Digimizer</t> 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).
    Computer Assisted Measurement Through Digimizer Image Analysis Software Version 4.2.2, supplied by MedCalc Software Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer-assisted+measurement+through+digimizer+image+analysis+software+version+4%2E2%2E2/computer+assisted+measurement+through+digimizer+image+analysis+software+version+4+2+2/pmc06372585-117-5-14
    Average 90 stars, based on 1 article reviews
    computer-assisted measurement through digimizer image analysis software version 4.2.2 - by Bioz Stars, 2026-09
    90/100 stars
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    Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed (Digimizer 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).

    Journal: Scientific Reports

    Article Title: Three Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease

    doi: 10.1038/s41598-019-38646-z

    Figure Lengend Snippet: Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed (Digimizer 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).

    Article Snippet: EM assessment was performed using computer-assisted measurement through Digimizer (version 4.2.2) image analysis software (MedCalc Software, Ostend, Belgium).

    Techniques: Transmission Assay, Electron Microscopy